DNA Extraction and Purification
Research Products International Corp Zyppy Plasmid Miniprep Kit, 400 Preps
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
Research Products International Corp ZymoPURE Plasmid Miniprep Kit, 50 Preps
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
ATCC Saccharomyces cerevisiae; genomic DNA
Genomic DNA isolated from Saccharomyces cerevisiae S288c. This fungal strain is also available as ATCC 204508.
Zymo Research Corporation DNA Clean & Concentrator™-25 (Capped) (50 Preps)
The DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 g DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Zymo Research Corporation DNA Degradase Plus™ (1000 U)
Since nucleosides lack negatively charged phosphate, DNA Degradase Plus™ is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a onestep procedure that is faster and simpler than other available methods. DNA Degradase Plus™ degrades DNA to individual nucleosides.Absence of the charged phosphate group facilitates quantitation via LC/MS. For applications compatible with nucleotides (e.g.- TLC, HPLC, etc.) see DNA Degradase (cat nos. E2016, E2017).
Zymo Research Corporation DNA Clean & Concentrator™-500 (20 Preps) w/ Zymo Spin VI
The DCC-500 features a single-buffer system that allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ VI Column. Simply add the specially formulated DNA Binding Buffer to your samples and transfer the mixtures to the supplied Zymo-Spin™ VI Column. The DNA is washed then eluted with a small volume ( 2 ml) of water or supplied DNA Elution Buffer using a centrifuge. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. The entire DNA purification/concentration procedure typically takes less than 20 minutes.
New England Biolabs, Inc. RNase H – 1250 units
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
New England Biolabs, Inc. NEBNext® Quick Ligation™ Module – 20 reactions
This module is part of the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 µg of input DNA vs. 500 pg - 1 µg required for the Ultra II DNA workflow. This module is also compatible with some Oxford Nanopore Technologies workflows.
- Compatible with some Oxford Nanopore Technologies workflows
Zymo Research Corporation Silicon-A™ Plate (2 Plates)
The Silicon-A Plate can be used in centrifuges for the large scale (i.e., 96-well) purification of DNA and/or RNA. Its lowprofile, durable polypropylene construction and unique silica-based matrix make it perfect for purifying up to 5 g DNA or RNA in 30 l eluate per well. Capacity is 600 l per well.
Zymo Research Corporation Zymoclean™ Large Fragment DNA Recovery Kit (25 Preps)
Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied ZymoSpin™ IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.
Zymo Research Corporation Quick-DNA/RNA Viral Kit (200 Preps)
The kit also features a buffer system that facilitates complete viral particle lysis for efficient nucleic acid isolation. Small (> 50 nt) and large (> 200 kb) DNA and RNA are bound to the column, washed and eluted. The isolated high-quality nucleic acids are ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/qPCR detection.
MilliporeSigma NUCLEOCLEAN DECONTAMINATION SP
Life Sciences,Immunodetection,NucleoClean Decontamination Spray
Enzo Life Sciences Human Genomic DNA, Male (100µg)
Human Genomic DNA, Male, is comprised of a pool of ≥ 8 normal donors. It is intact DNA extracted from freshly harvested whole blood of healthy males. DNA is treated with DNase free-RNase to remove residual contaminant RNA. Human genomic DNA can be used for a variety of molecular applications including PCR, southern blotting, and DNA library construction. It can also be used as normal reference DNA for comparative genomic hybridizations (CGH). Alternative name: gDNA. Concentration: 150 - 250 µg/mL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA. Formulation: Liquid. Long Term Storage: -20°C.
Magbio Genomics Inc HighPrep PCR (250mL)
HighPrep™ PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.