DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Lupus: Heart, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
New England Biolabs, Inc. Monarch® Spin PCR & DNA Cleanup Kit - 250 preps
The Monarch Spin PCR & DNA Cleanup Kit (5 ug), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 ug of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 ul, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps
Zymo Research Corporation EZ-96 DNA Methylation™ Kit (Shallow-Well) (2 x 96 Rxns)
The EZ-96 DNA MethylationTM Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The products innovative desulphonation technology eliminates otherwise cumbersome precipitations.The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation ZymoPURE™ P3 (Yellow) (210 ml)
ZymoPURE™ P3 (Yellow) (210 ml). For use in ZymoPURE™ Plasmid Purification kits. Colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Research Products International Corp Quick-DNA Fungal/Bacterial DNA MicroPrep Kit, 50 Preps
The Quick-DNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.
Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
Simple: Fastest workflow (≤ 20 minutes).
Biochain Institute Inc Genomic DNA Extraction Kit, 1 kit/PK
BioChain's DNA extraction kit is a convenient tool for isolating high quality genomic DNA. The isolated DNA can be used for PCR amplification template, Southern Blot analysis, SNP analysis, DNA methylation research, etc.. The kit contains enough reagents for isolating genomic DNA from 5 gram tissue and cells.
Biochain Institute Inc Serum DNA Isolation Kit, 1 kit/PK
BioChain's Serum DNA Isolation kit offers a fast, efficient, and convenient alternative to traditional phenol or protease treatment. Our protocol guarantees isolate DNA from 200 ul serum or plasma samples. In less than 15 minutes, users can obtain pure DNA ready for analysis by PCR, sequencing, or microarrays.
Takara Bio NUCLEOSNAP PLASMID MIDI 50PREP
ndotoxins are copurified during plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Macherey-Nagel has developed a new column format and a novel buffer chemistry to enable vacuum-processed isolation of transfection-grade plasmid DNA in a midiprep format. TAKARA BIO USA INC mfg# 740494.50
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Stomach, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation EZ-96 DNA Methlyation-Direct™ Kit (Shallow-Well) (2 x 96 Rxns)
The increased sensitivity of this kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold™ Kit, DNA denaturation and bisulfite conversion processes are combined into a single step. All kits streamline the three step process of bisulfite conversion of nonmethylated cytosine in DNA into uracil. In addition the methylation kits share innovative in-column desulphonation technology that eliminates otherwise cumbersome DNA precipitation steps while ensuring researchers consistent results every time. The kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
Braintree Scientific Accuris Mouse Genotyping Kit, 1 hour, mammalian,80 reactions
Accuris™ 1 Hour Mouse/Mammalian Genotyping Kit DNA extraction and amplification in 1 hour Optimize proprietary lysis buffer Single tube - no organic solvents or clean up procedures Enhanced Accuris Hot Start Polymerase included The Accuris Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR Accuris Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers. **Sold for research purposes only and not for human use**
Macherey-Nagel NucleoBond Xtra Maxi, 10 preps
NucleoBond Xtra Maxi (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A
Beckman Coulter COULTER AGENCOURT GENFIND V3 R
Coulter Agencourt GenFind V3 Reagent Kit - 50 Preps Product No