DNA Extraction and Purification
Research Products International Corp Zymo ZymoBIOMICS-96 DNA MagBead Kit
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Research Products International Corp Genomic DNA Clean & Concentrator Kit-25, 100 Preps
The Genomic DNA Clean & Concentrator-25 (DCC) is for quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Bufferto a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)DNA, etc.) from anyenzymatic reaction or impure preparation (e.g., Proteinase K digestion). No messy precipitations!
Research Products International Corp Oligo Clean & Concentrator, 200 Preps
The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥ 16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure. DNA/RNA is washed and concentrated into a small volume of water. Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
Fast & Simple: Clean and concentrate DNA and RNA oligonucleotides in 2 minutes.
Ultra-Pure: Ready for hybridization, Next-Gen Sequencing, PCR, ligations, etc.
Complete removal: Eliminate dyes, salts, enzymes, nucleotides, and short oligos.
Biochain Institute Inc Genomic DNA Extraction Kit, 1 kit/PK
BioChain's DNA extraction kit is a convenient tool for isolating high quality genomic DNA. The isolated DNA can be used for PCR amplification template, Southern Blot analysis, SNP analysis, DNA methylation research, etc.. The kit contains enough reagents for isolating genomic DNA from 5 gram tissue and cells.
Biochain Institute Inc Blood and Serum DNA Isolation Kit, 1 kit/PK
BioChain's DNA extraction kit is a convenient tool for isolating high quality genomic DNA. The isolated DNA can be used for PCR amplification template, Southern Blot analysis, SNP analysis, and DNA methylation research. Each kit can be used to isolate genomic DNA from 5 grams of tissue and cells.
Biochain Institute Inc Express Cloning Checker Kit II: Blue Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Biochain Institute Inc Express Cloning Checker Kit I: Yellow Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Biochain Institute Inc Express Cloning Checker Kit II: Green Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Biochain Institute Inc Stool DNA Isolation Kit, 1 kit/PK
BioChain's stool DNA extraction kit is a convenient and easy to use means of extracting high purity genomic DNA from stool samples. This kit does not require organic extraction with toxic reagents such as phenol or chloroform, or loss-inducing precipitation steps. The lysis-binding and wash buffers are formulated to remove contaminants that may inhibit downstream applications, such as next generation sequencing, PCR, and methylation studies that are key to a better understanding of gastrointestinal cancers such as colorectal cancer.
New England Biolabs, Inc. NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® – 96 reactions
The NEBNext Ultra II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, and the NEBNext Ultra II Q5 Master Mix for uniform, high-fidelity library amplification.
- Perform fragmentation, end repair and dA-tailing with a single enzyme mix
- Experience reliable fragmentation with a single protocol, regardless of input amount or GC content
- Prepare high quality libraries from a wide range of input amounts: 100 pg-500 ng
- Use with DNA in standard buffers (TE, Tris-HCl) and water